Dominic Calvert-Lewin, Bukayo Saka and Harvey Barnes have been handed their first senior England call-ups, while Mason Greenwood and Phil Foden have been dropped.
After hitting a career-best 15 goals last term, Calvert-Lewin’s impressive start to the new campaign has propelled Everton to three wins from three in the Premier League.
The 23-year-old is the Premier League’s joint-leading goalscorer with five, and also scored a hat-trick for the Toffees in Wednesday’s Carabao Cup win over West Ham.
Saka, 19, has featured just three times for Arsenal during this campaign but broke into the first-team and impressed while playing at full-back for the Gunners last season.
Barnes meanwhile has helped Leicester to the top of the early Premier League table, with the 22-year-old scoring in the Foxes’ win over Burnley.
As expected, Southgate has left out Greenwood and Foden after the pair were sent home from Iceland last month for breaching coronavirus protocols by meeting up with two women inside the public areas of the team hotel.
Manchester United captain Harry Maguire has been recalled, after he was dropped from the last squad following his convictions for assault and attempted bribery in a Greek court, which he has since appealed with the convictions nullified ahead of a full retrial.
Having handed first-time call-ups to six players for last month’s matches, Southgate has a more experienced squad this time, with fit-again Ben Chilwell, Harry Winks and Marcus Rashford returning to the fold.
Liverpool captain Jordan Henderson is also named in the squad despite picking up an injury issue in the Reds’ win over Chelsea.
England have three home games during this international break, including Nations League games against Belgium and Denmark and a friendly against Wales.
England squad in full
Goalkeepers: Dean Henderson (Manchester United), Nick Pope (Burnley), Jordan Pickford (Everton).
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Chelsea), Conor Coady (Wolves), Eric Dier (Tottenham), Joe Gomez (Liverpool), Michael Keane (Everton), Harry Maguire (Manchester United), Ainsley Maitland-Niles (Arsenal), Tyrone Mings (Aston Villa), Bukayo Saka (Arsenal), Kieran Trippier (Atletico Madrid), Kyle Walker (Manchester City).
Midfielders: Jordan Henderson (Liverpool), Mason Mount (Chelsea), Kalvin Phillips (Leeds United), Declan Rice (West Ham), James Ward-Prowse (Southampton), Harry Winks (Tottenham).
Forwards: Tammy Abraham (Chelsea), Harvey Barnes (Leicester), Dominic Calvert-Lewin (Everton), Jack Grealish (Aston Villa), Danny Ings (Southampton), Harry Kane (Tottenham), Marcus Rashford (Manchester United), Jadon Sancho (Borussia Dortmund), Raheem Sterling (Manchester City).
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